蓖麻凝集素与线粒体内膜的结合是制备过程中的一种假象。
Binding of Ricinus communis agglutinin to the mitochondrial inner membrane as an artifact during preparation.
作者信息
Köhle H, Kauss H
出版信息
Biochem J. 1979 Dec 15;184(3):721-3. doi: 10.1042/bj1840721.
Abstract
Endosperm from Ricinus communis was homogenized in the presence of 3H-labelled Ricinus communis agglutinin, with or without addition of lactose. In preparations without the binding-specific sugar the subfraction containing the mitochondrial inner membrane contained sufficient labelled agglutinin to account for the agglutinin reported to be associated with this membrane.
摘要
在有或没有添加乳糖的情况下，将蓖麻的胚乳在3H标记的蓖麻凝集素存在下进行匀浆。在没有结合特异性糖的制剂中，含有线粒体内膜的亚组分含有足够的标记凝集素，足以解释据报道与该膜相关的凝集素。
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