Porteous J W
Environ Health Perspect. 1979 Dec;33:25-35. doi: 10.1289/ehp.793325.
The anatomy of the intestine is outlined. The kinetics of cell proliferation, differentiation, and migration in intestinal epithelium are described. The distribution of cell types between the proliferation and differentiation compartments of the epithelium is summarized. Seven preparations of intestine in current use for metabolic studies are listed. An outline review is presented of recent results on glucose, amino acid and ketone body metabolism obtained with three of these preparations: the ligature-isolated loop of intestine in vivo; the vascularly and luminally perfused intestine in vitro; and suspensions of respiring intestinal epithelial cells in vitro. Reasons are given for thinking that these experimental systems are among the most useful currently available for metabolic studies, perhaps especially in the investigation of the metabolic fate of potentially toxic materials to which animal species may be exposed.
概述了肠道的解剖结构。描述了肠道上皮细胞增殖、分化和迁移的动力学。总结了上皮细胞增殖和分化区室之间细胞类型的分布。列出了目前用于代谢研究的七种肠道制剂。对使用其中三种制剂获得的葡萄糖、氨基酸和酮体代谢的最新结果进行了概述性综述：体内结扎分离的肠袢；体外血管灌注和腔内灌注的肠道；以及体外呼吸的肠上皮细胞悬液。给出了认为这些实验系统是目前代谢研究中最有用的系统之一的理由，也许在研究动物物种可能接触的潜在有毒物质的代谢命运方面尤其如此。
