Mayahara T, Suzuki K, Ohata H, Nakamura T, Yamada S
Nihon Yakurigaku Zasshi. 1979 Jul;75(5):419-23. doi: 10.1254/fpj.75.419.
An automatic adder mixer for photometric cells was newly developed in our laboratory and was applied in experiments of superprecipitation of myosin B. The mixer has a reciprocating blade-type impeller with a pit for loading samples. The pit was cut so as to be just above the meniscus of the solution in a cell at the top dead point of the cam mechanism and so as to enter the solution at the bottom dead point. When 10 microliter of methylene blue solution was added, the mixing time was 0.6 sec. Myosin B was extracted from rabbit white muscle. Adding ATP (1 mM) to myosin B suspension and mixing was carried out by hand, using a mixing plunger, and also using the automatic adder mixer. The recordings obtained were then compared. Continuous recording of the initial reaction was successfully obtained only when the automatic adder mixer was used. Excellent results were also obtained with respect to reproducibility. The automatic adder mixer is easy to make, inexpensive, and can be utilized in conjunction with conventional spectrophotometers.
我们实验室新开发了一种用于光度池的自动加样混合器，并将其应用于肌球蛋白B的超沉淀实验。该混合器有一个带用于装载样品凹槽的往复叶片式叶轮。凹槽的切割位置设计为在凸轮机构的上死点时刚好位于细胞内溶液弯月面上方，并在下死点时进入溶液。加入10微升亚甲蓝溶液时，混合时间为0.6秒。肌球蛋白B从兔白肌中提取。向肌球蛋白B悬浮液中加入ATP（1 mM）并进行混合，手工操作时使用混合柱塞，同时也使用自动加样混合器。然后比较得到的记录。只有使用自动加样混合器时才能成功获得初始反应的连续记录。在重现性方面也取得了优异的结果。该自动加样混合器易于制作、成本低廉，并且可以与传统分光光度计结合使用。
