远紫外线下蛋白质和肽的吸收
Absorption of proteins and peptides in the far ultraviolet.
作者信息
Woods A H, O'Bar P R
出版信息
Science. 1970 Jan 9;167(3915):179-81. doi: 10.1126/science.167.3915.179.
PMID:5409641
Abstract
An absorption peak for the peptide bond at 187 nanometers has been confirmed; a protein assay at this wavelength allows quantitation of proteins in aqueous solution at concentrations between 0.1 and 25 micrograms per milliliter. Assays are conducted at 6 degrees C to take advantage of the reduction of end-absorption of water with temperature.
摘要
已确认肽键在187纳米处有一个吸收峰；在此波长下进行蛋白质测定，能够对浓度在每毫升0.1至25微克之间的水溶液中的蛋白质进行定量分析。测定在6摄氏度下进行，以利用水的末端吸收随温度降低的特性。
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