Slifkin M, Merkow L P, Pardo M, Epstein S M, Leighton J, Farber E
Science. 1970 Jan 16;167(3916):285-7. doi: 10.1126/science.167.3916.285.
Cell suspensions obtained from hyperplastic nodules induced in rat liver by either of the two hepatic carcinogens, 2-fluorenylacetamide or aflatoxin B(1), show growth when cultured in vitro. No growth of cells from liver adjacent to the hyperplastic nodules or from liver of control rats has been obtained so far under comparable conditions. Hepatocarcinoma cells induced by 2-fluorenylacetamide grow readily in vitro but behave differently. These findings suggest that some nonmalignant cells capable of growth in vitro arise during liver carcinogenesis prior to the appearance of unequivocal cancer. Cultures of such cells may offer new avenues for the study of liver carcinogenesis.
从由两种肝癌致癌物（2-芴基乙酰胺或黄曲霉毒素B1）之一诱导大鼠肝脏产生的增生性结节中获得的细胞悬液，在体外培养时会生长。到目前为止，在类似条件下，未获得来自增生性结节附近肝脏或对照大鼠肝脏的细胞生长。由2-芴基乙酰胺诱导的肝癌细胞在体外易于生长，但表现不同。这些发现表明，在明确的癌症出现之前，肝脏致癌过程中会产生一些能够在体外生长的非恶性细胞。此类细胞的培养可能为肝癌发生的研究提供新途径。
