Craig I W, Gibor A
J Cell Biol. 1970 Feb;44(2):305-9. doi: 10.1083/jcb.44.2.305.
The chloroplasts of internodal cells of Nitella are fixed to a stationary layer of cytoplasm whereas the nuclei and most of the cytoplasm stream along the longitudinal axis. Isolated internodal cells were maintained for several days with half the cell kept in the dark, the other half kept under continuous light. Photosynthetic activity of the cells was checked by placing the cell evenly illuminated in a (14)CO(2) atmosphere. Chloroplasts of the previously dark half of the cell were found to fix only half as much CO(2) as the chloroplasts which were continuously illuminated. These results are discussed in relation to the possible direct effect of light on biosynthetic reactions of mature chloroplasts.
丽藻节间细胞的叶绿体固定在一层静止的细胞质上，而细胞核和大部分细胞质则沿纵轴流动。将分离的节间细胞培养数天，细胞的一半置于黑暗中，另一半置于连续光照下。通过将细胞均匀光照于（14）CO₂气氛中来检测细胞的光合活性。发现细胞先前处于黑暗中的那一半的叶绿体固定的CO₂量仅为持续光照的叶绿体的一半。结合光对成熟叶绿体生物合成反应可能的直接影响对这些结果进行了讨论。
