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通过扫描电子显微镜研究鸡胚晚期原条和头突。

The chick embryo late primitive streak and head process studied by scanning electron microscopy.

作者信息

Wakely J, England M A

出版信息

J Anat. 1979 Oct;129(Pt 3):615-22.

PMID:541244
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1233027/
Abstract

Changes in cell shape during the formation of the head process and regression of Hensen's node have been examined by scanning electron microscopy in a series of chick embryos from stage -5 to stage -8. The endoderm was removed from embryos mounted for New culture so that the mesoderm could be viewed from the ventral surface. As the primitive streak shortens its cells flatten and lose the flap-like processes characteristic of earlier stages. The notochord differentiates and its cells become transversely orientated, then re-align along the embryonic axis between stage 6 and stage -8. At the same time a split or cleft appears between notochord and head mesoderm, and extracellular fibrils are formed which are the first sign of the formation of a notochordal sheath.

摘要

通过扫描电子显微镜,对一系列处于-5期至-8期的鸡胚进行观察,研究了头部突起形成过程中以及亨氏结退化过程中细胞形状的变化。将用于新培养的胚胎的内胚层去除,以便从腹面观察中胚层。随着原条缩短,其细胞变平并失去早期阶段特有的片状突起。脊索开始分化,其细胞呈横向排列,然后在6期至-8期之间沿胚胎轴重新排列。与此同时,在脊索和头部中胚层之间出现了一条裂缝,并且形成了细胞外纤维,这是脊索鞘形成的第一个迹象。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/941b91f3bfff/janat00239-0167-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/57291fdd8e5f/janat00239-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/9ac37b9b6984/janat00239-0165-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/4dcdab9b7da9/janat00239-0165-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/250a4867b4cc/janat00239-0165-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/1c9a018b1d00/janat00239-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/5a2e452f8f20/janat00239-0166-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/f4471fae3f8b/janat00239-0166-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/2c4844f6c552/janat00239-0166-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/9f94cb0e8a23/janat00239-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/941b91f3bfff/janat00239-0167-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/57291fdd8e5f/janat00239-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/9ac37b9b6984/janat00239-0165-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/4dcdab9b7da9/janat00239-0165-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/250a4867b4cc/janat00239-0165-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/1c9a018b1d00/janat00239-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/5a2e452f8f20/janat00239-0166-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/f4471fae3f8b/janat00239-0166-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/2c4844f6c552/janat00239-0166-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/9f94cb0e8a23/janat00239-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/1233027/941b91f3bfff/janat00239-0167-b.jpg

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本文引用的文献

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J Morphol. 1965 May;116:357-69. doi: 10.1002/jmor.1051160304.
2
The development of the notochord in chick embryos.鸡胚胎中脊索的发育。
J Embryol Exp Morphol. 1962 Dec;10:602-21.
3
The cell coat in the early chick embryo.早期鸡胚中的细胞被膜。
Anat Embryol (Berl). 1974;146(2):181-92. doi: 10.1007/BF00315594.
4
Avian gastrulation.鸟类原肠胚形成。
Adv Morphog. 1971;9:231-62. doi: 10.1016/b978-0-12-028609-6.50010-8.
5
The development of the notochord in the chick embryo, studied by scanning and transmission electron microscopy.通过扫描电子显微镜和透射电子显微镜对鸡胚中脊索的发育进行研究。
J Embryol Exp Morphol. 1976 Apr;35(2):383-401.
6
Scanning electron microscopy of the development of the mesoderm layer in chick embryos.鸡胚中胚层发育的扫描电子显微镜观察
Anat Embryol (Berl). 1977 May 12;150(3):291-300. doi: 10.1007/BF00318347.
7
Hensen's node - the 'organizer' of the amniote embryo.亨氏结——羊膜动物胚胎的“组织者”。
Experientia. 1976 Mar 15;32(3):269-77. doi: 10.1007/BF01940787.
8
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Anat Embryol (Berl). 1978 Jun 2;153(2):167-78. doi: 10.1007/BF00343372.
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