离子载体灰黄霉素、白黄霉素及其两种衍生物对大鼠肝线粒体中钾离子依赖性谷氨酸外流的比较作用。

Comparative effects of ionophores grisorixin, alborixin and two derivatives on K+ glutamate efflux in rat liver mitochondria.

作者信息

Chapel M, Jeminet G, Gachon P, Debise R, Durand R

出版信息

J Antibiot (Tokyo). 1979 Jul;32(7):740-5. doi: 10.7164/antibiotics.32.740.

DOI:10.7164/antibiotics.32.740

Abstract

The alkali cations discrimination on a liquid membrane electrodes system, was determined for the carboxylic ionophores grisorixin, alborixin and two derivatives, dihydrogrisorixin and hexahydroalborixin. The two antibiotics exhibited a great perference for K+. Dihydrogrisorixin again showed the selectivity curve of a carboxylic ionophore, but with a discrimination power lowered compared with grisorixin. Hexahydroalborixin had lost all the complexing properties of the natural molecule. The selectivity scales measured for cations, were directly correlated with the K+ and glutamate effluxes measured in rat liver mitochondria. The chemical modifications of the natural structures of grisorixin and alborixin resulted in a drastic reduction of their ionophoric properties. The loss of K+-glutamate might occur in two steps, the efflux of K+ catalysed by the ionophores then causing a loss of negative charges in the form of glutamate.

摘要

在液膜电极系统中，对羧酸离子载体灰黄霉素、白硼霉素以及两种衍生物二氢灰黄霉素和六氢白硼霉素进行了碱金属阳离子鉴别。这两种抗生素对K⁺表现出强烈的偏好。二氢灰黄霉素再次呈现出羧酸离子载体的选择性曲线，但与灰黄霉素相比，其鉴别能力有所降低。六氢白硼霉素已丧失天然分子的所有络合特性。所测得的阳离子选择性系数与在大鼠肝线粒体中测得的K⁺和谷氨酸外流直接相关。灰黄霉素和白硼霉素天然结构的化学修饰导致其离子载体性能急剧下降。K⁺ - 谷氨酸的流失可能分两步发生，离子载体催化K⁺外流，进而导致以谷氨酸形式的负电荷流失。