Zombola R, Bearse R C, Kitos P A
J Cell Physiol. 1979 Oct;101(1):57-65. doi: 10.1002/jcp.1041010108.
The concentration of trace elements in L-cells has been studied as a function of the trace metal content of the growth medium. Cells were cultured in synthetic media which contained varying trace amounts of the elements manganese, iron, cobalt, copper, zinc and molybdenum. The cellular concentration of the of the elements potassium, iron, copper and zinc were then determined. It was found that the cell accumulates trace metals at a different rate than they are made available. Deficiencies in zinc could be "induced" in the cell by increasing the concentration of iron, manganese and cobalt; cellular iron deficiencies were observed at larger medium concentrations of zinc, manganese, copper and cobalt. Trace metal uptake by the cell was seen to parallel the utilization by multicellular organisms.
已经研究了L细胞中微量元素的浓度与生长培养基中微量金属含量的关系。细胞在含有不同微量的锰、铁、钴、铜、锌和钼元素的合成培养基中培养。然后测定细胞中钾、铁、铜和锌元素的浓度。发现细胞积累微量金属的速率与它们可利用的速率不同。通过增加铁、锰和钴的浓度可在细胞中“诱导”锌缺乏；在锌、锰、铜和钴浓度较高的培养基中观察到细胞铁缺乏。细胞对微量金属的摄取与多细胞生物的利用情况相似。
