Barbieri B, Abbruzzi R, Benigni A, Rizzardini M, Donelli M G, Garattini S, Salmona M
J Chromatogr. 1979 Jun 11;163(2):195-200. doi: 10.1016/s0378-4347(00)81463-2.
A thin-layer chromatographic method has been developed for the detection and measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and its major metabolite trifluoroacetyladriamycin (AD 41). The procedure gives satisfactory linearity over a large range of concentrations. The coefficient of variability is about 10% over the entire range of usable concentrations, giving good reproducibility; sensitivity is 25 ng for both AD 32 and AD 41. Analysis is specific for AD 32 and AD 41 since adriamycin or more polar metabolites can be differentiated. Recovery is high (85-90%) and the method is simple and economical to use. Pharmacokinetics of AD 32 and AD 41 are reported in blood and some tissues of mice bearing Lewis Lung carcinoma.
已开发出一种薄层色谱法，用于检测和测定N-三氟乙酰阿霉素-14-戊酸酯（AD 32）及其主要代谢产物三氟乙酰阿霉素（AD 41）。该方法在很大的浓度范围内具有令人满意的线性。在整个可用浓度范围内，变异系数约为10%，具有良好的重现性；AD 32和AD 41的灵敏度均为25 ng。该分析对AD 32和AD 41具有特异性，因为阿霉素或极性更强的代谢产物可以被区分。回收率很高（85-90%），且该方法使用简单、经济。报道了AD 32和AD 41在荷Lewis肺癌小鼠的血液和一些组织中的药代动力学。
