Maiorino R M, Sipes I G, Gandolfi A J, Brown B R
J Chromatogr. 1979 Sep 11;164(1):63-72. doi: 10.1016/s0378-4347(00)81572-8.
A simple and sensitive gas chromatographic method for the determination of 2-chloro-1, 1-difluoroethylene (CDE) and 2-chloro-1,1,1-trifluoroethane (CTE), two highly volatile metabolites of halothane, in blood, liver and isolated hepatic microsomes is described. The entire head-space in equilibrium with a known volume or weight of the sample is injected into the gas chromatograph equipped with a flame ionization detector. Quantification is accomplished with standards prepared by fortifying blank samples with known concentrations of CDE and CTE which are treated under the same conditions as the samples. Detection limits for CDE and CTE were 2 pmole/ml in blood and 10 pmole/g in liver and the mean relative standard deviations are no greater than +/- 6% except for CTE in hepatic microsomes (+/- 9%). A preliminary study of blood CDE and CTE levels in humans anesthetized with halothane is reported.
本文描述了一种简单且灵敏的气相色谱法，用于测定血液、肝脏及离体肝微粒体中氟烷的两种高挥发性代谢物——2-氯-1,1-二氟乙烯（CDE）和2-氯-1,1,1-三氟乙烷（CTE）。将与已知体积或重量的样品达到平衡的整个顶空部分注入配备火焰离子化检测器的气相色谱仪中。通过向空白样品中加入已知浓度的CDE和CTE制备标准品，并在与样品相同的条件下进行处理来完成定量分析。CDE和CTE在血液中的检测限为2皮摩尔/毫升，在肝脏中的检测限为10皮摩尔/克，除肝微粒体中的CTE（±9%）外，平均相对标准偏差不大于±6%。本文还报道了对接受氟烷麻醉的人体血液中CDE和CTE水平的初步研究。
