Cumming D F
Biochem J. 1970 Jan;116(2):189-98. doi: 10.1042/bj1160189.
The nucleotides present in the cambial tissue (primary wall tissue) and in the not yet fully differentiated secondary xylem (secondary wall tissue) of Larix decidua Mill. were extracted and characterized. The method of extraction best suited to the material was investigated and the problems involved in desalting of extracts and their effect on the final nucleotide pattern obtained are discussed. UDP-glucose was found to be the most important sugar nucleotide isolated from both cambial and young xylem tissue. UDP-galactose, UDP-arabinose, UDP-xylose, UDP-fructose, GDP-glucose, GDP-galactose, GDP-mannose, ADP-glucose, ADP-galactose, ADP-fructose, ADP-ribose and ADP-ribose phosphate (the last two compounds are the acid breakdown products of NADH and NADPH respectively) were also found in both extracts. UDP-galacturonic acid was identified only in the extract of the primary wall material. Several nucleotide oligosaccharides were obtained from both the extract of cambial tissue and that of the young xylem.
对欧洲落叶松形成层组织(初生壁组织)和尚未完全分化的次生木质部(次生壁组织)中的核苷酸进行了提取和表征。研究了最适合该材料的提取方法,并讨论了提取物脱盐过程中涉及的问题及其对最终获得的核苷酸模式的影响。发现UDP-葡萄糖是从形成层和幼嫩木质部组织中分离出的最重要的糖核苷酸。在两种提取物中还发现了UDP-半乳糖、UDP-阿拉伯糖、UDP-木糖、UDP-果糖、GDP-葡萄糖、GDP-半乳糖、GDP-甘露糖、ADP-葡萄糖、ADP-半乳糖、ADP-果糖、ADP-核糖和ADP-核糖磷酸(最后两种化合物分别是NADH和NADPH的酸分解产物)。UDP-半乳糖醛酸仅在初生壁材料的提取物中被鉴定出来。从形成层组织提取物和幼嫩木质部提取物中均获得了几种核苷酸寡糖。