Akhrem A A, Avvakumov G V, Sidorova I V, Strel'chyonok O A
J Chromatogr. 1979 Nov 28;180(1):69-82. doi: 10.1016/s0021-9673(00)80175-2.
A technique for the gas chromatographic analysis of the products of solvolysis of permethylated glycopeptides and glycoproteins has been developed. It involves methanolysis of a permethylated compound, quantitative transformation of methyl ethers of methyl glycosides into the corresponding O-trimethylsilyl(TMS)-O-methylalditols [2-deoxy-2-(N-methyl)acetamido-O-TMS-O-methylalditols in the case of hexosamine derivatives] and gas chromatographic quantification using a single column packed with 0.4% OV-225 on surface-modified Chromosorb.
已开发出一种用于全甲基化糖肽和糖蛋白溶剂解产物气相色谱分析的技术。该技术包括全甲基化化合物的甲醇解、甲基糖苷的甲基醚定量转化为相应的O-三甲基硅烷基（TMS）-O-甲基糖醇[对于己糖胺衍生物，为2-脱氧-2-（N-甲基）乙酰胺基-O-TMS-O-甲基糖醇]以及使用填充有0.4% OV-225的表面改性Chromosorb单柱进行气相色谱定量分析。
