Muñoz G, Bongiorni-Malavé I
J Exp Zool. 1979 Nov;210(2):253-7. doi: 10.1002/jez.1402100208.
Eggs were recovered from 153 adult female mice fed either a normal (27% protein) or a protein-deficient (8% protein) diet from day 15 (Group I) or 30 (Group II) before mating until sacrifice. The females were mated with fertile males and sacrificed 48, 72, 96 or 120 hours after observation of vaginal plugs. The study was performed with females that ovulated spontaneously and with females induced to ovulate by injection of pregnant mare's serum gonadotrophin and human chorionic gonadotrophin. Without gonadotrophin treatment, significantly fewer eggs were recovered from malnourished than from normal females. However, after hormonal treatment, the number of eggs ovulated by malnourished and normal females increased similarly. About 50% of the eggs recovered from malnourished females in both groups I and II were cleaved whereas 90% of the eggs recovered from well-nourished females were cleaved. The development of the cleaved eggs from protein deficient females was delayed and asynchronous in comparison with that of eggs from normal females. Embryos recovered from malnourished females also were retarded in differentiation of morulae to blastocysts. It is concluded that a protein-restricted diet adversely affects the fertilization of eggs as well as their early development and differentiation.
从153只成年雌性小鼠中收集卵子，这些小鼠在交配前从第15天（第一组）或第30天（第二组）开始，要么喂食正常饮食（蛋白质含量27%），要么喂食蛋白质缺乏饮食（蛋白质含量8%），直至处死。雌性小鼠与可育雄性小鼠交配，并在观察到阴栓后48、72、96或120小时处死。该研究对自然排卵的雌性小鼠以及通过注射孕马血清促性腺激素和人绒毛膜促性腺激素诱导排卵的雌性小鼠进行。未经促性腺激素处理时，营养不良的雌性小鼠回收的卵子明显少于正常雌性小鼠。然而，激素处理后，营养不良和正常雌性小鼠排卵的数量增加程度相似。第一组和第二组中从营养不良雌性小鼠回收的卵子约50%发生了卵裂，而从营养良好雌性小鼠回收的卵子90%发生了卵裂。与正常雌性小鼠的卵子相比，蛋白质缺乏雌性小鼠的卵裂卵发育延迟且不同步。从营养不良雌性小鼠回收的胚胎在从桑椹胚分化为囊胚的过程中也受到阻碍。结论是，蛋白质限制饮食对卵子的受精及其早期发育和分化有不利影响。
