分离的心肌细胞的自发节律性收缩。

Spontaneous rhythmic contraction of separated heart muscle cells.

作者信息

Bloom S

出版信息

Science. 1970 Mar 27;167(3926):1727-9. doi: 10.1126/science.167.3926.1727.

DOI:10.1126/science.167.3926.1727

Abstract

Muscle cells that conitract spontaneously and rhythmically can be obtained from adult mouse myocardium. Contractions are observed immediately after homogenization in a solution that is ionically similar to intracellular fluid. Contraction frequency varies directly with temperature and decreases as a function of time after homogenization. At 16 degrees C rhythmic relaxation and contraction occur for about 20 minutes. Contractions are dependent on the presence of adenosine triphosphate in the homogenization medium.

摘要

能够自发且有节律地收缩的肌肉细胞可以从成年小鼠的心肌中获取。在与细胞内液离子相似的溶液中匀浆后，立即就能观察到收缩现象。收缩频率与温度直接相关，并随着匀浆后时间的推移而降低。在16摄氏度时，有节律的舒张和收缩会持续约20分钟。收缩依赖于匀浆介质中三磷酸腺苷的存在。