Ciplea A G, Bock P R, von Schmude D, Pollock B
Arzneimittelforschung. 1975 Oct;25(10):1552-7.
A fast and simple method is described for the localisation of tips of chronically implanted electrodes in the brain of rabbits which may be obtained--under standard conditions--by an intra-aortal injection of a 2% potassium-hexacyanoferrate-III solution. This method also facilitates histological preparations with a thickness of 5 mu only and thus permits a thorough morpho-pathological examination not only in the area of the electrode tips but in adjacent zones as well. The use of a buffered formalin solution also permits certain histochemical examinations to be made, for instance the determination of nucleic acids.
本文描述了一种快速简便的方法,用于定位长期植入兔脑内电极的尖端。在标准条件下,通过主动脉内注射2%的铁氰化钾溶液即可实现。该方法还便于制作仅5微米厚的组织切片,从而不仅可以对电极尖端区域,还可以对相邻区域进行全面的形态病理学检查。使用缓冲福尔马林溶液还可以进行某些组织化学检查,例如核酸的测定。