采用高效液相色谱法测定大鼠组织中硫胺素及其磷酸酯的浓度。

Concentrations of thiamine and its phosphate esters in rat tissues determined by high-performance liquid chromatography.

作者信息

Ishii K, Sarai K, Sanemori H, Kawasaki T

出版信息

J Nutr Sci Vitaminol (Tokyo). 1979;25(6):517-23. doi: 10.3177/jnsv.25.517.

DOI:10.3177/jnsv.25.517

Abstract

Thiamine and its phosphate esters in rat tissues were determined by high-performances liquid chromatography after conversion to thiochrome and its phosphate esters as described previously (Ishii, K. et al. (1979) Anal. Biochem., 97, 191--195). Total thiamine concentrations determined in the brain, liver, heart and kidney were 6.39, 25.5, 15.7, and 14.9 nmol/g wet weight, respectively. In all the tissues tested, thiamine, its monophosphate, pyrophosphate, and triphosphate were found to be present at the rates of 1.2--7.7, 5.9--11.0, 85.7--90.0, and 0.7--1.6%, respectively. Although these percentages of thiamine phosphates were similar to those reported previously using different procedures, the percentage of thiamine triphosphate content in rat tissues was found to be fairly lower than that reported. The time required for the whole process of analysis averaged 90 min after isolation of the organs from the animal.

摘要

如前所述（石井，K.等人（1979年）《分析生物化学》，97，191 - 195），将大鼠组织中的硫胺素及其磷酸酯转化为硫色素及其磷酸酯后，通过高效液相色谱法进行测定。在脑、肝、心和肾中测定的总硫胺素浓度分别为6.39、25.5、15.7和14.9 nmol/g湿重。在所有测试组织中，发现硫胺素、其一磷酸酯、二磷酸酯和三磷酸酯的存在比例分别为1.2 - 7.7%、5.9 - 11.0%、85.7 - 90.0%和0.7 - 1.6%。尽管这些硫胺素磷酸酯的百分比与先前使用不同方法报道的相似，但发现大鼠组织中硫胺素三磷酸酯的含量百分比比报道的要低得多。从动物体内分离出器官后，整个分析过程所需的平均时间为90分钟。