哺乳动物组织中磷酸硫胺素酯的含量——猪骨骼肌中硫胺素三磷酸的浓度极高。

Content of thiamin phosphate esters in mammalian tissues--an extremely high concentration of thiamin triphosphate in pig skeletal muscle.

作者信息

Egi Y, Koyama S, Shikata H, Yamada K, Kawasaki T

出版信息

Biochem Int. 1986 Mar;12(3):385-90.

Abstract

In relation to a high activity of thiamin diphosphate (TDP) kinase (Koyama, S. et al. (1985) Biochem. Int. 11, 371-380) in the skeletal muscle of pigs and guinea pigs, the content of thiamin phosphate esters in tissues of these animals has been determined by the method of high-performance liquid chromatography. An extremely high concentration of thiamin triphosphate (TTP), 69.2% of the total thiamin (26.1 nmol/g wet weight), was detected in adult pig skeletal muscles. One extreme case contained TTP as 88.7% of the total thiamin (19.6 nmol/g wet weight). TTP in pig skeletal muscle was found solely in cytosol fraction. This is the first report showing an unusually high level of TTP in mammals and may give a clue as to the physiological functions of TTP.

摘要

鉴于猪和豚鼠骨骼肌中硫胺素二磷酸（TDP）激酶具有较高活性（小山，S.等人（1985年），《生物化学国际》11，371 - 380），已采用高效液相色谱法测定了这些动物组织中硫胺素磷酸酯的含量。在成年猪骨骼肌中检测到极高浓度的硫胺素三磷酸（TTP），占总硫胺素的69.2%（26.1纳摩尔/克湿重）。有一个极端案例中，TTP占总硫胺素的88.7%（19.6纳摩尔/克湿重）。猪骨骼肌中的TTP仅存在于胞质溶胶部分。这是首次报道哺乳动物中TTP水平异常高，可能为TTP的生理功能提供线索。