Chan K C, Leung O C, Lee L H
Microbios. 1979;24(96):81-91.
Cells of the moderately halophilic Micrococcus varians var. halophilus grew well in a chemically defined medium containing 1 to 3 M NaCl and 0.0103 M K+. The requirement for NaCl could be partially replaced by K+,:Li+ and Cs+. The efficiency of the sparing effect of these cations for NaCl was in order of K+ GReater than Li+ greater than Cs+. Increase in growth temperature was found to enchance the sparing effect of Li+ and Cs+ but not that of K+. Over the range of NaCl concentrations in which the cells grew well, cell-Na+ concentrations were similar to the medium NaCl concentrations while cellK+ concentrations were several-fold that in the medium. Cell-bound Na+ and K+ concentrations increased proportionally with medium NaCl concentration and growth temperature. The temperature-dependent cation accumulation was more obvious with K+ than Na+. The cell-associated Na+ + K+ concentrations were almost as high as or slightly higher than the external media which contained appropriate levels of NaCl regardless of the growth temperature.
中度嗜盐的嗜盐变异微球菌（Micrococcus varians var. halophilus）细胞在含有1至3 M氯化钠和0.0103 M钾离子的化学限定培养基中生长良好。氯化钠的需求可部分被钾离子、锂离子和铯离子替代。这些阳离子对氯化钠的替代效应效率顺序为：钾离子＞锂离子＞铯离子。发现生长温度升高会增强锂离子和铯离子的替代效应，但不会增强钾离子的替代效应。在细胞生长良好的氯化钠浓度范围内，细胞内钠离子浓度与培养基中氯化钠浓度相似，而细胞内钾离子浓度是培养基中钾离子浓度的几倍。细胞结合的钠离子和钾离子浓度随培养基中氯化钠浓度和生长温度成比例增加。钾离子的温度依赖性阳离子积累比钠离子更明显。无论生长温度如何，细胞相关的钠离子 + 钾离子浓度几乎与含有适当水平氯化钠的外部培养基一样高或略高。
