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[对鸡胚腺垂体促肾上腺皮质激素细胞研究的新组织化学贡献]

[New histochemical contributions to the study of corticotropic cells in the adenohypophysis of the chick enbryo].

作者信息

Ferrand R, Herlant M, Dessy C

出版信息

C R Acad Hebd Seances Acad Sci D. 1975 Jul 7;281(1):57-60.

PMID:54227
Abstract

Comparative analysis of results obtained after subsequent treatments of chick embryo adenohypophysis by anti-ACTH 17-39 Is, PAS and Bodian method demonstrates that ACTH cells are PAS positive, Bodian negative and are located in the cephalic lobe. With the histochemical fluorescence method of Falck, the cells marked by anti-ACTH 17-39 Is exhibit an induced fluorescence, after L-DOPA injection. However, after treatment by metopirone, numerous cells of the caudal part of the adenohypophysis take up L-DOPA but they are not corticotrophs.

摘要

用抗促肾上腺皮质激素17 - 39免疫血清、过碘酸雪夫(PAS)法和博迪安(Bodian)法对鸡胚腺垂体进行后续处理后所获得结果的比较分析表明,促肾上腺皮质激素细胞呈PAS阳性、博迪安阴性,且位于头叶。采用法尔克(Falck)组织化学荧光法,注射左旋多巴后,用抗促肾上腺皮质激素17 - 39免疫血清标记的细胞呈现诱导荧光。然而,用甲吡酮处理后,腺垂体尾部的许多细胞摄取左旋多巴,但它们不是促肾上腺皮质激素细胞。

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