Førre O, Ly B, Thorsteinsson L, Hovig T
Scand J Haematol. 1979 Nov;23(5):381-7. doi: 10.1111/j.1600-0609.1979.tb02737.x.
The peripheral blood cells, spleen cells and bone marrow cells from a patient with hairy cell leukaemia were studied by means of several immunological methods and by phase contrast and electron microscopy. Both by light and electron microscopy the cells had the morphology of hairy cells. 60 % of all the peripheral blood cells, and 80 % of the spleen cells had membrane-bound IgGk immunoglobulin. 60 % of the peripheral blood lymphocytes and 90 % of the spleen cells were positive for Ia-antigens, and 80 % of the peripheral blood, and 70 % of the spleen cells had receptors for complement factor C3. The percentages of cells with receptor for the Fc part of IgC and receptors for sheep red blood cells (SRBC) were low both in peripheral blood and in spleen. Reduced numbers of peroxidase positive cells and cytotoxic plaque-forming cells were also observed as well as reduced lymphocyte responses after stimulation of peripheral blood lymphocytes with PHA, PWM, ConA, PPD and allogeneic cells. A normal antibody-dependent cell cytotoxicity (ADCC) and PHA-induced cytotoxicity was observed for the peripheral blood lymphocytes of the patients. Our results suggest that the hairy cells in our patient are derived from B lymphocytes and have a monoclonal origin.
采用多种免疫学方法以及相差显微镜和电子显微镜，对一名毛细胞白血病患者的外周血细胞、脾细胞和骨髓细胞进行了研究。通过光学显微镜和电子显微镜观察，细胞均具有毛细胞的形态。所有外周血细胞的60%以及脾细胞的80%具有膜结合IgGk免疫球蛋白。外周血淋巴细胞的60%和脾细胞的90%Ia抗原呈阳性，外周血的80%以及脾细胞的70%具有补体因子C3受体。外周血和脾脏中具有IgC的Fc部分受体以及绵羊红细胞（SRBC）受体的细胞百分比均较低。还观察到过氧化物酶阳性细胞和细胞毒性噬斑形成细胞数量减少，以及用PHA、PWM、ConA、PPD和同种异体细胞刺激外周血淋巴细胞后淋巴细胞反应降低。观察到患者外周血淋巴细胞具有正常的抗体依赖性细胞毒性（ADCC）和PHA诱导的细胞毒性。我们的结果表明，我们患者的毛细胞源自B淋巴细胞，并且具有单克隆起源。
