Leighton T J, Stock J J, Kelln R A
J Bacteriol. 1970 Aug;103(2):439-46. doi: 10.1128/jb.103.2.439-446.1970.
Biochemical events which occur during macroconidial germination have been studied in the dermatophyte Microsporum gypseum. The specific activity levels of various metabolic enzymes have been assayed during germination time periods. The accumulated levels of several of these enzymes, as a function of exogenous carbohydrate source, have been investigated. M. gypseum was found to possess a constitutive glyoxalate shunt, a constitutive glucokinase, a fructose phosphoenolpyruvate transferase, and a mannitol phosphoenolpyruvate transferase. The integration of endogenous reserve utilization during germination is discussed. The purification and properties of an alkaline phosphatase and its possible relationship to sporulation and spore germination also are described.
已对皮肤癣菌石膏样小孢子菌大分生孢子萌发过程中发生的生化事件进行了研究。在萌发时间段内测定了各种代谢酶的比活性水平。已研究了其中几种酶的累积水平与外源碳水化合物来源的关系。发现石膏样小孢子菌具有组成型乙醛酸循环支路、组成型葡萄糖激酶、果糖磷酸烯醇丙酮酸转移酶和甘露醇磷酸烯醇丙酮酸转移酶。讨论了萌发过程中内源性储备利用的整合情况。还描述了一种碱性磷酸酶的纯化及其性质，以及它与孢子形成和孢子萌发可能的关系。
