HORIKOSHI K, IIDA S, IKEDA Y
J Bacteriol. 1965 Feb;89(2):326-30. doi: 10.1128/jb.89.2.326-330.1965.
Horikoshi, Koki (The Institute of Physical and Chemical Research, Tokyo, Japan), Shigeji Iida, and Yonosuke Ikeda. Mannitol and mannitol dehydrogenases in conidia of Aspergillus oryzae. J. Bacteriol. 89:326-330. 1965.-A sugar alcohol was isolated from the conidia of Aspergillus oryzae and identified as d-mannitol. Two types of d-mannitol dehydrogenases, nicotinamide adenine dinucleotide phosphate-linked and nicotinamide adenine dinucleotide-linked, were found in the conidia. Substrate specificities, pH optima, Michaelis-Menton constants, and the effects of inhibitors were studied. d-Mannitol was converted to fructose by the dehydrogenases. Synthesis of d-mannitol dehydrogenases was not observed during germination; the content of d-mannitol decreased at an early stage of germination. It was assumed, therefore, that d-mannitol might be used as the source of endogenous respiration and provide energy for the germination.
堀越浩史（日本东京理化研究所）、饭田重次和池田米之助。米曲霉分生孢子中的甘露醇及甘露醇脱氢酶。《细菌学杂志》89:326 - 330。1965年。——从米曲霉分生孢子中分离出一种糖醇并鉴定为d - 甘露醇。在分生孢子中发现了两种类型的d - 甘露醇脱氢酶，即烟酰胺腺嘌呤二核苷酸磷酸连接型和烟酰胺腺嘌呤二核苷酸连接型。研究了底物特异性、最适pH值、米氏常数及抑制剂的作用。脱氢酶将d - 甘露醇转化为果糖。在萌发过程中未观察到d - 甘露醇脱氢酶的合成；d - 甘露醇含量在萌发早期下降。因此推测，d - 甘露醇可能作为内源性呼吸的来源并为萌发提供能量。
