Shen P Y, Coles E, Foote J L, Stenesh J
J Bacteriol. 1970 Aug;103(2):479-81. doi: 10.1128/jb.103.2.479-481.1970.
The fatty acid distribution of three mesophilic and three thermophilic strains of the genus Bacillus was determined by gas chromatography of the fatty acid methyl esters. Fatty acid i-15:0 was the most abundant in both the mesophiles (51%) and the thermophiles (41%). The second most abundant fatty acid was a-15:0 in the mesophiles (22%), and i-17:0 in the thermophiles (27%). The fatty acid pair i-15:0, i-17:0 was the most predominant pair in both the mesophiles (61%) and the thermophiles (66%). The fatty acid pair a-15:0, a-17:0 was the second most predominant pair and was much higher in the mesophiles (30%) than in the thermophiles (15%). The average fatty acid chain length was 15.5 for the mesophiles and 16.0 for the thermophiles. The significance of these results for the lipid theory of thermophily is discussed.
通过脂肪酸甲酯的气相色谱法测定了芽孢杆菌属的三株嗜温菌和三株嗜热菌的脂肪酸分布。脂肪酸i-15:0在嗜温菌(51%)和嗜热菌(41%)中都是含量最丰富的。含量第二丰富的脂肪酸在嗜温菌中是a-15:0(22%),在嗜热菌中是i-17:0(27%)。脂肪酸对i-15:0、i-17:0在嗜温菌(61%)和嗜热菌(66%)中都是最主要的脂肪酸对。脂肪酸对a-15:0、a-17:0是第二主要的脂肪酸对,在嗜温菌(30%)中比在嗜热菌(15%)中含量高得多。嗜温菌的平均脂肪酸链长度为15.5,嗜热菌的平均脂肪酸链长度为16.0。讨论了这些结果对嗜热脂质理论的意义。