Nakano N, Nagahara A, Shimizu T, Aibara K, Fujimoto Y, Morooka N, Tatsuno T
Jpn J Med Sci Biol. 1979 Oct;32(5):269-79. doi: 10.7883/yoken1952.32.269.
The distribution in the mouse tissues of 13-[14C]-12,13-epoxtrichothec-9-ene administered intravenously was determined by whole-body autoradiography and by tracing the radioactivity of the tissues oxidized in an Auto Sample Oxidizer. The appearance of the label in urine and feces was also followed by the tracer technique. The distributions of radioactivity in tissues as determined by the two methods were almost identical. On the autoradiograms of mice killed 10 min after the injection, marked blackening of the film was observed at the sites corresponding to the liver, kidney, and bladder with urine, and much less darkening at other sites. The radioactivities contained in the liver, kidney, urine and small intestine were 13.3, 2.3, 2.6 and 10.2% of the dose, respectively. The labeled toxin was rapidly excreted into urine and feces, 56.0 and 4.9% in 6 hr and 66.7 and 28.0% in 24 hr after injection, respectively. Oral administration of the labeled toxin to mother mice resulted in the appearance of radioactivity in the stomach contents of 7-day suckling mice, thus demonstrating indirectly the secretion of the toxin into the milk. An attempt to show a respiratory route of excretion in rats given the radioactive compound orally or intravenously failed to detect any radioactivity in the expired CO2 collected for 6 hr, suggesting that the 14C in the epoxy ring was intact.
通过全身放射自显影以及追踪自动样品氧化器中氧化的组织的放射性，测定了静脉注射13-[¹⁴C]-12,13-环氧单端孢霉烯-9-烯在小鼠组织中的分布。还采用示踪技术追踪了尿液和粪便中标记物的出现情况。通过这两种方法测定的组织中放射性分布几乎相同。在注射后10分钟处死的小鼠的放射自显影片上，在与肝脏、肾脏以及含尿液的膀胱相对应的部位观察到胶片明显变黑，而其他部位变黑程度小得多。肝脏、肾脏、尿液和小肠中所含的放射性分别为剂量的13.3%、2.3%、2.6%和10.2%。标记毒素迅速排泄到尿液和粪便中，注射后6小时分别为56.0%和4.9%，24小时分别为66.7%和28.0%。给母鼠口服标记毒素后，在7日龄哺乳小鼠的胃内容物中出现了放射性，从而间接证明了毒素分泌到乳汁中。对口服或静脉注射放射性化合物的大鼠进行排泄的呼吸途径的研究，未能在收集6小时的呼出二氧化碳中检测到任何放射性，这表明环氧环中的¹⁴C是完整的。
