高铁血红素的聚集。原高铁血红素和次高铁血红素在水溶液中的二聚作用及质子解离平衡。
Aggregation of ferrihaems. Dimerization and protolytic equilibria of protoferrihaem and deuteroferrihaem in aqueous solution.
作者信息
Brown S B, Dean T C, Jones P
出版信息
Biochem J. 1970 May;117(4):733-9. doi: 10.1042/bj1170733.
Abstract
- The absorption spectra of deutero- and proto-ferrihaem in aqueous solution at 25 degrees C show marked changes with concentration and pH in the Soret band region. Quantitative studies of these phenomena imply that they are associated with ferrihaem dimerization and with protolytic equilibria involving monomeric (M) and dimeric (D) ferrihaem species according to the scheme: [Formula: see text] 2. For deuteroferrihaem we obtain K=1.9x10(-2), pK(a(M))=7.1, pK(a(D))=7.4. Protoferrihaem has a much higher dimerization constant, K=4.5 and pK(a(D))=7.5 (pK(a(M)) is not accessible). 3. Possible structural relationships between monomeric and dimeric ferrihaem species in solution are discussed in relation to recent work on the oxo-bridged nature of crystalline ferrihaem dimers.
摘要
- 25摄氏度下，水溶液中氘代高铁血红素和原高铁血红素的吸收光谱在索雷特带区域随浓度和pH值呈现显著变化。对这些现象的定量研究表明，它们与高铁血红素二聚化以及涉及单体（M）和二聚体（D）高铁血红素物种的质子解离平衡有关，具体过程如下：[公式：见原文] 2. 对于氘代高铁血红素，我们得到K = 1.9×10⁻²，pK(a(M)) = 7.1，pK(a(D)) = 7.4。原高铁血红素有更高的二聚化常数，K = 4.5且pK(a(D)) = 7.5（pK(a(M))无法测得）。3. 结合近期关于晶体高铁血红素二聚体氧桥性质的研究工作，讨论了溶液中单体和二聚体高铁血红素物种之间可能的结构关系。
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