Redburn D A, Thomas T N
J Neurosci Methods. 1979 Oct;1(3):235-42. doi: 10.1016/0165-0270(79)90034-7.
Homogenization of rabbit retina in isotonic sucrose and differential rate centrifugation yielded two morphologically distinct synaptosomal fractions. One fraction was enriched in photoreceptor cell synaptosomes; the second fraction contained small synaptosomes derived from conventional sized synapses most abundant in the inner plexiform layer. Attempts to further purify these fractions using a variety of density gradients proved unsuccessful due to poor viability of photoreceptor cell synaptosomes. The synaptosomes prepared by our method are functionally stable as they demonstrate high affinity uptake for putative retinal neurotransmitters, neurotransmitter-sensitive adenylate cyclase activity, and calcium-dependent, potassium-stimulated release of [14C]GABA and [3H]dopamine.
将兔视网膜在等渗蔗糖中匀浆并进行差速离心，得到了两个形态上不同的突触体组分。一个组分富含光感受器细胞突触体；第二个组分包含源自内网状层中最丰富的常规大小突触的小突触体。由于光感受器细胞突触体的活力较差，尝试使用各种密度梯度进一步纯化这些组分未成功。我们的方法制备的突触体在功能上是稳定的，因为它们对假定的视网膜神经递质表现出高亲和力摄取、神经递质敏感的腺苷酸环化酶活性，以及钙依赖性、钾刺激的[14C]GABA和[3H]多巴胺释放。
