Shreiner D P, Levin J
J Clin Invest. 1970 Sep;49(9):1709-13. doi: 10.1172/JCI106388.
Rabbits in which thrombocytosis had been produced by five daily transfusions of platelet concentrates had suppressed endogenous thrombopoiesis, as reflected by decreased incorporation of selenomethionine-(75)Se ((75)SeM) into the circulating platelet mass. Rabbits in which endogenous thrombopoiesis had been suppressed by transfusion-induced thrombocytosis were used to detect thrombopoietic activity in rabbit plasma. Thrombopoietic activity was demonstrated in the plasma of both normal and thrombocytopenic donor rabbits. A dose response relationship was observed between the incorporation of (75)SeM into platelets and the dose of plasma administered. Infusion of 20-150 ml of plasma from thrombocytopenic donors increased the incorporation of (75)SeM into platelets from 52 to 107% above control values. A dose response effect also was seen after infusion of normal plasma, but normal plasma produced less effect than comparable doses of plasma from thrombocytopenic donors. Rabbits with transfusion-induced thrombocytosis appear to be more sensitive assay animals for the detection of thrombopoietic activity than animals with normal platelet counts. Changes in the rate of appearance and levels of (75)SeM may primarily indicate changes in platelet protein or platelet size and are apparently more sensitive indicators of the state of thrombopoiesis than are alterations in the numbers of circulating platelets. The results strongly support the concept of a humoral agent, i.e. thrombopoietin, that acts on megakaryocytes to regulate platelet production.
通过连续五天输注血小板浓缩物而产生血小板增多症的兔子，其内源性血小板生成受到抑制，这可通过硒代蛋氨酸 -（75）硒（（75）SeM）掺入循环血小板量的减少来反映。通过输血诱导的血小板增多症抑制了内源性血小板生成的兔子被用于检测兔血浆中的血小板生成活性。在正常供体兔和血小板减少症供体兔的血浆中均证实了血小板生成活性。观察到（75）SeM掺入血小板与所给予血浆剂量之间存在剂量反应关系。输注20 - 150毫升血小板减少症供体的血浆可使（75）SeM掺入血小板的量比对照值增加52%至107%。输注正常血浆后也观察到剂量反应效应，但正常血浆产生的效应小于相同剂量的血小板减少症供体血浆。与血小板计数正常的动物相比，输血诱导的血小板增多症的兔子似乎是检测血小板生成活性更敏感的实验动物。（75）SeM出现速率和水平的变化可能主要表明血小板蛋白质或血小板大小的变化，并且显然是比循环血小板数量变化更敏感的血小板生成状态指标。这些结果有力地支持了一种体液因子即血小板生成素的概念，它作用于巨核细胞以调节血小板生成。
