多线染色体中的膨化现象与组蛋白乙酰化
Puffing and histone acetylation in polytene chromosomes.
作者信息
Clever U, Ellgaard E G
出版信息
Science. 1970 Jul 24;169(3943):373-4. doi: 10.1126/science.169.3943.373.
PMID:5450368
Abstract
Fixation with picric acid or formaldehyde retains incorporated [(3)H]acetate which is lost after fixation with ethanol and acetic acid. Unlike [(3)H]uridine, [(3)H]acetate is diffusely incorporated into polytene chromosomes, and not preferentially into existing or newly induced puffs. It is suggested that puff formation does not include an acetylation of histones.
摘要
用苦味酸或甲醛固定可保留掺入的[³H]乙酸盐，而用乙醇和乙酸固定后该物质会丢失。与[³H]尿苷不同，[³H]乙酸盐会分散地掺入多线染色体中，而不是优先掺入现有的或新诱导的胀泡中。这表明胀泡形成不包括组蛋白的乙酰化。
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