Bell W R, Regoeczi E
J Clin Invest. 1970 Oct;49(10):1872-9. doi: 10.1172/JCI106406.
The kinetics of the depletion of plasma fibrinogen were studied in seven patients who received fibrinogen-(131)I 1 hr before an intravenous injection of the coagulating enzyme (CE) derived from the venom of the pit viper, Agkistrodon rhodostoma. Disappearance of the clottable radioactivity labeled fibrinogen from the circulation conformed to an exponential decay with an average half-life of 0.85 hr. The mean clearance rate for protein-bound radioactivity, composed of fibrinogen and it's split products, was 12% of the intravascular pool per hour. The breakdown products of fibrin produced by CE inhibited polymerization of fibrin in vitro. Studies in five patients performed between the 3rd and 10th day following the administration of CE revealed that the absolute catabolic rates of fibrinogen were subnormal initially, but gradually increased as the fibrinogen concentration returned to normal. In rabbits, after the administration of CE, regeneration of the fibrinogen pool was markedly prolonged. This delayed regeneration time was not influenced by an excess of antivenene, but rapid regeneration to pretreatment values of plasma fibrinogen was immediately initiated by stimulating fibrinogen synthesis with subcutaneous turpentine.
在7名患者中研究了血浆纤维蛋白原消耗的动力学，这些患者在静脉注射源自红口蝮蛇毒液的凝血酶（CE）前1小时接受了纤维蛋白原 -（131）I。循环中可凝结的放射性标记纤维蛋白原的消失符合指数衰减，平均半衰期为0.85小时。由纤维蛋白原及其裂解产物组成的蛋白质结合放射性的平均清除率为每小时血管内池的12%。CE产生的纤维蛋白降解产物在体外抑制纤维蛋白的聚合。在给予CE后的第3天至第10天对5名患者进行的研究表明，纤维蛋白原的绝对分解代谢率最初低于正常水平，但随着纤维蛋白原浓度恢复正常而逐渐增加。在兔子中，给予CE后，纤维蛋白原池的再生明显延长。这种延迟的再生时间不受过量抗蛇毒血清的影响，但通过皮下注射松节油刺激纤维蛋白原合成可立即使血浆纤维蛋白原迅速再生至预处理值。
