Prior B A, Fennema O, Marth E H
Appl Microbiol. 1970 Jul;20(1):139-44. doi: 10.1128/am.20.1.139-144.1970.
VARIOUS BACTERIA, YEASTS, AND MOLDS IMPORTANT TO THE FOOD INDUSTRY WERE INCUBATED IN AEROSOL CANS CONTAINING A C BROTH AND ONE OF THE FOLLOWING THREE GAS HYDRATE FORMERS: propane, dichlorodifluoromethane (f-12), and 1,1-difluoro-1-chloroethane (f-142b). Most hydrate formers were tested at three concentrations: low (vapor state), intermediate (liquid state, low level), and high (liquid state, high level). Samples were continuously agitated for 48 hr at 21 +/- 3 C. Changes in numbers of microorganisms were determined by plate count. With hydrate formers in the vapor state, propane was more toxic to the microorganisms tested than either f-12 or f-142b. The most resistant organisms from these trials were then tested against f-12 or f-142b in the liquid state. Hydrate formers were far more toxic in the liquid state than in the vapor state. With the exception of sporulated cultures of Bacillus cereus, all microorganisms tested were greatly reduced in numbers when agitated for 48 hr at 21 C in the presence of f-12 or f-142b.
将对食品工业至关重要的各种细菌、酵母菌和霉菌置于装有A C肉汤以及以下三种气体水合物形成剂之一的气雾剂罐中培养：丙烷、二氯二氟甲烷（F - 12）和1,1 - 二氟 - 1 - 氯乙烷（F - 142b）。大多数水合物形成剂在三种浓度下进行测试：低浓度（气态）、中等浓度（液态，低水平）和高浓度（液态，高水平）。样品在21±3℃下持续搅拌48小时。通过平板计数确定微生物数量的变化。对于气态的水合物形成剂，丙烷对所测试的微生物的毒性比F - 12或F - 142b更强。然后将这些试验中最具抗性的生物体针对液态的F - 12或F - 142b进行测试。水合物形成剂在液态时的毒性远比气态时大。在21℃下，在F - 12或F - 142b存在的情况下搅拌48小时后，除蜡样芽孢杆菌的芽孢形成培养物外，所有测试的微生物数量都大幅减少。
