锌离子对正常人红细胞血红蛋白氧亲和力及2,3-二磷酸甘油酸水平的体外影响。

Effect of zinc ions on the oxygen affinity of hemoglobin and the level of 2,3-DPG in normal human red blood cells in vitro.

作者信息

Kabat A I, Niedworok J, Kedziora J, Błaszczyk J, Bartosz G

出版信息

Zentralbl Bakteriol B. 1979;169(5-6):436-45.

Abstract

This paper presents the results of experiments on the in vitro effect of zinc ions on the affinity of hemoglobin for oxygen and on the 2,3-DPG content of the red blood cells of healthy humans. Blood was incubated in 1.5 X 10(-3) M ZnSO4 solution for 4 h and then the oxygen affinity of hemoglobin and the hematocrit number were determined. An original method of determination the oxygen dissociation curve of HbO2 was employed enabling an exact calculation of the P50 value of the hemolysates by the method of least squares. Analysis of statistically verified results reveals that zinc ions increase the oxygen affinity of hemoglobin bringing about a leftward shift of the HbO2 dissociation curve (by 3.85 mm Hg for P50) the level of 2,3-DPG remaining constant in the red cells incubated with ZnSO4. The mechanisms of this phenomenon and its possible clinical implications are discussed.

摘要

本文介绍了锌离子对健康人红细胞血红蛋白氧亲和力及2,3 - 二磷酸甘油酸（2,3 - DPG）含量的体外影响实验结果。血液在1.5×10⁻³ M硫酸锌（ZnSO₄）溶液中孵育4小时，然后测定血红蛋白的氧亲和力和血细胞比容值。采用一种测定血红蛋白氧解离曲线的原始方法，通过最小二乘法能够精确计算溶血产物的P50值。对经统计学验证的结果分析表明，锌离子增加了血红蛋白的氧亲和力，导致血红蛋白氧解离曲线向左移动（P50降低3.85 mmHg），而在与ZnSO₄孵育的红细胞中2,3 - DPG水平保持不变。本文讨论了这一现象的机制及其可能的临床意义。