Boyne A W, Duncan W R
J Lipid Res. 1970 Jul;11(4):293-300.
A computer program is described which analyzes output punched directly onto paper tape from a gas-liquid chromatograph. Although this program was written specifically for samples of fatty acid methyl esters derived from adipose tissue triglycerides which are eluted within 1 hr, modification of the dimension statements in the program would enable it to deal with samples which require a longer time to come off the column. The salient features of the rationale of the program are discussed in detail, particularly the procedures for base line correction and for estimating the contributions from components which are not perfectly separated in the column. Examples are given of the program in practice, of comparing the results it gives with those obtained by manual triangulation of the areas on a recorder chart, and of indicating the range of column load over which we have found that it operates satisfactorily. A sample computer print-out from the program is presented and interpreted.
本文描述了一个计算机程序，该程序可分析直接从气-液色谱仪打印在纸带上的输出。虽然此程序是专门为从脂肪组织甘油三酯衍生而来的脂肪酸甲酯样品编写的，这些样品在1小时内被洗脱，但修改程序中的维度语句将使其能够处理需要更长时间从色谱柱流出的样品。详细讨论了该程序原理的显著特点，特别是基线校正程序以及估计色谱柱中未完全分离的组分的贡献的程序。给出了该程序在实际应用中的示例，将其给出的结果与通过手动三角测量记录器图表上的面积获得的结果进行比较，并指出我们发现该程序运行令人满意的柱负载范围。还给出并解释了该程序的一份示例计算机打印输出。
