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无需提取的组织脂质薄层色谱法。

Thin-layer chromatography of tissue lipids without extraction.

作者信息

Cherayil G D, Scaria K S

出版信息

J Lipid Res. 1970 Jul;11(4):378-81.

PMID:5459668
Abstract

Frozen tissue sections were applied directly to silica gel plates, and the lipids were separated by developing the plates in different solvent systems. Quantitation of the lipid classes was achieved by direct transmission densitometry after the plates were treated with a chromic acid-sulfuric acid spray.

摘要

将冷冻组织切片直接铺在硅胶板上,通过在不同溶剂系统中展开硅胶板来分离脂质。在用铬酸 - 硫酸喷雾处理硅胶板后,通过直接透射密度测定法定量脂质类别。

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