绵羊红细胞的冷冻保存。2. 纯化的聚乙烯吡咯烷酮和水解淀粉作为保护剂。

Cryopreservation of sheep red blood cells. 2. Purified polyvinylpyrrolidone and hydrolyzed starch as protective agents.

作者信息

Myhrvold V

出版信息

Acta Vet Scand. 1979;20(4):531-6. doi: 10.1186/BF03546580.

DOI:10.1186/BF03546580

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8322911/

Abstract

The protection of sheep erythrocytes against damage at low temperature (−196°C) was investigated using purified polyvinylpyrrolidone (PVP) and hydrolyzed starch as cryoprotective agents. Identical results were obtained with untreated PVP, neutralized PVP and PVP purified by chromatography. With hydrolyzed starch the cryoprotection was dependent on the type and concentration of the starch used and on the extent of hydrolysis of the starch prior to use. Cell recovery with some starch types was the same (80–85 %) as that obtained with PVP.

摘要

使用纯化的聚乙烯吡咯烷酮（PVP）和水解淀粉作为冷冻保护剂，研究了绵羊红细胞在低温（-196°C）下免受损伤的情况。未经处理的PVP、中和的PVP和通过色谱法纯化的PVP得到了相同的结果。对于水解淀粉，冷冻保护作用取决于所用淀粉的类型和浓度以及使用前淀粉的水解程度。某些淀粉类型的细胞回收率与PVP相同（80-85%）。

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3

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