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[利用自动图像处理对白化大鼠17区进行细胞形态计量学分析]

[Cytomorphometry of area 17 of the albino rat using the automatic picture processing].

作者信息

Werner L, Voss K

出版信息

J Hirnforsch. 1979;20(5):527-37.

PMID:546982
Abstract

Using the automatical picture processing, the layers II to IV of the adult albino-rat's area 17 were investigated in Nisselpreparations. Within 15 hours 14 features from approximately 10 000 neurons could be registered. Five features (neuron density = neuron number per 10 areas of measurement, mean value of neuron area in picture points, mean value of mean extinctions of neurons, whole extinctions of neurons, mean value of relative granula area of neurons) were statistically calculated and considered under several aspects: 1st Concerning the specifity of the layers very exact results could be obtained. 2nd In a detailed investigation of the lamina V pyramidal cells estimating somata areas no statistically significant classification could be achieved. 3rd There are slight structural changes of area 17 in rostrocaudal direction, but without statistical significance. Further investigations are necessary. 4th It is possible to differentiate between pyramidal cells and stellate cells of lamina IV. The numerical proportion between these two types of cells, however, deviates from individual estimations. Until now, this problem could not be satisfactorily be solved.

摘要

利用自动图像处理技术,在尼氏染色标本中对成年白化大鼠17区的II至IV层进行了研究。在15小时内,可记录来自约10000个神经元的14个特征。对五个特征(神经元密度=每10个测量区域的神经元数量、图像点中神经元面积的平均值、神经元平均消光值、神经元整体消光值、神经元相对颗粒面积的平均值)进行了统计计算,并从几个方面进行了考量:第一,关于各层的特异性,可获得非常精确的结果。第二,在对V层锥体细胞的体细胞面积进行详细研究时,未能实现具有统计学意义的分类。第三,17区在前后方向存在轻微的结构变化,但无统计学意义。需要进一步研究。第四,有可能区分IV层的锥体细胞和星状细胞。然而,这两种细胞类型之间的数量比例与个体估计值存在偏差。到目前为止,这个问题尚未得到令人满意的解决。

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