龙虾神经中三羧酸循环与乙酰胆碱合成的研究

Cheng S C, Nakamura R

Biochem J. 1970 Jul;118(3):451-5. doi: 10.1042/bj1180451.

The pattern of metabolism of (14)C-labelled substrates in the lobster nerve suggested a normal tricarboxylic acid cycle with a slow turnover. 2. Acetylcholine was synthesized from [2-(14)C]acetate, [2-(14)C]pyruvate and [1,5-(14)C]citrate, implying the presence of acetate thiokinase, choline acetylase and citrate-cleavage enzyme. 3. [2-(14)C]Acetate was the best precursor. 4. The formation of acetyl-CoA from citrate was limited, probably by the citrate-cleavage enzyme, although the magnitude of the reversed reactions of the tricarboxylic acid cycle was large when compared with that of the forward reactions. 5. The relative magnitude of the two pathways (acetyl-CoA and carbon dioxide fixation) in pyruvate utilization was nearly equal. 6. The probable presence of metabolic compartments in the lobster nerve is discussed.

龙虾神经中（14）C标记底物的代谢模式表明存在一个周转缓慢的正常三羧酸循环。2. 乙酰胆碱由[2-（14）C]乙酸盐、[2-（14）C]丙酮酸和[1,5-（14）C]柠檬酸盐合成，这意味着存在乙酸硫激酶、胆碱乙酰转移酶和柠檬酸裂解酶。3. [2-（14）C]乙酸盐是最佳前体。4. 尽管与正向反应相比，三羧酸循环逆向反应的幅度较大，但由柠檬酸盐形成乙酰辅酶A受到限制，这可能是由于柠檬酸裂解酶的作用。5. 丙酮酸利用中两条途径（乙酰辅酶A和二氧化碳固定）的相对幅度几乎相等。6. 讨论了龙虾神经中可能存在的代谢区室。

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核心技术专利：CN118964589B侵权必究

Cheng S C, Nakamura R

Biochem J. 1970 Jul;118(3):451-5. doi: 10.1042/bj1180451.

The pattern of metabolism of (14)C-labelled substrates in the lobster nerve suggested a normal tricarboxylic acid cycle with a slow turnover. 2. Acetylcholine was synthesized from [2-(14)C]acetate, [2-(14)C]pyruvate and [1,5-(14)C]citrate, implying the presence of acetate thiokinase, choline acetylase and citrate-cleavage enzyme. 3. [2-(14)C]Acetate was the best precursor. 4. The formation of acetyl-CoA from citrate was limited, probably by the citrate-cleavage enzyme, although the magnitude of the reversed reactions of the tricarboxylic acid cycle was large when compared with that of the forward reactions. 5. The relative magnitude of the two pathways (acetyl-CoA and carbon dioxide fixation) in pyruvate utilization was nearly equal. 6. The probable presence of metabolic compartments in the lobster nerve is discussed.

龙虾神经中（14）C标记底物的代谢模式表明存在一个周转缓慢的正常三羧酸循环。2. 乙酰胆碱由[2-（14）C]乙酸盐、[2-（14）C]丙酮酸和[1,5-（14）C]柠檬酸盐合成，这意味着存在乙酸硫激酶、胆碱乙酰转移酶和柠檬酸裂解酶。3. [2-（14）C]乙酸盐是最佳前体。4. 尽管与正向反应相比，三羧酸循环逆向反应的幅度较大，但由柠檬酸盐形成乙酰辅酶A受到限制，这可能是由于柠檬酸裂解酶的作用。5. 丙酮酸利用中两条途径（乙酰辅酶A和二氧化碳固定）的相对幅度几乎相等。6. 讨论了龙虾神经中可能存在的代谢区室。