龙虾神经中三羧酸循环与乙酰胆碱合成的研究
A study on the tricarboxylic acid cycle and the synthesis of acetylcholine in the lobster nerve.
作者信息
Cheng S C, Nakamura R
出版信息
Biochem J. 1970 Jul;118(3):451-5. doi: 10.1042/bj1180451.
Abstract
- The pattern of metabolism of (14)C-labelled substrates in the lobster nerve suggested a normal tricarboxylic acid cycle with a slow turnover. 2. Acetylcholine was synthesized from [2-(14)C]acetate, [2-(14)C]pyruvate and [1,5-(14)C]citrate, implying the presence of acetate thiokinase, choline acetylase and citrate-cleavage enzyme. 3. [2-(14)C]Acetate was the best precursor. 4. The formation of acetyl-CoA from citrate was limited, probably by the citrate-cleavage enzyme, although the magnitude of the reversed reactions of the tricarboxylic acid cycle was large when compared with that of the forward reactions. 5. The relative magnitude of the two pathways (acetyl-CoA and carbon dioxide fixation) in pyruvate utilization was nearly equal. 6. The probable presence of metabolic compartments in the lobster nerve is discussed.
摘要
- 龙虾神经中（14）C标记底物的代谢模式表明存在一个周转缓慢的正常三羧酸循环。2. 乙酰胆碱由[2-（14）C]乙酸盐、[2-（14）C]丙酮酸和[1,5-（14）C]柠檬酸盐合成，这意味着存在乙酸硫激酶、胆碱乙酰转移酶和柠檬酸裂解酶。3. [2-（14）C]乙酸盐是最佳前体。4. 尽管与正向反应相比，三羧酸循环逆向反应的幅度较大，但由柠檬酸盐形成乙酰辅酶A受到限制，这可能是由于柠檬酸裂解酶的作用。5. 丙酮酸利用中两条途径（乙酰辅酶A和二氧化碳固定）的相对幅度几乎相等。6. 讨论了龙虾神经中可能存在的代谢区室。
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本文引用的文献
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SOME QUANTITATIVE ASPECTS OF THE FIXATION OF CARBON DIOXIDE BY THE LOBSTER NERVE.龙虾神经对二氧化碳固定作用的一些定量方面
Biochem Z. 1963;338:643-53.
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Some observations on the effect of D-glutamate on the glucose metabolism and the accumulation of potassium ions in brain cortex slices.关于D-谷氨酸对大脑皮层切片中葡萄糖代谢及钾离子积累影响的一些观察
J Neurochem. 1959 Jun;4(2):124-34. doi: 10.1111/j.1471-4159.1959.tb13181.x.
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Effect of ions on the efflux of acetylcholine from peripheral nerve.离子对乙酰胆碱从外周神经流出的影响。
J Gen Physiol. 1966 Nov;50(2):447-60. doi: 10.1085/jgp.50.2.447.
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Relative contribution of carbon dioxide fixation and acetyl-coA pathways in two nervous tissues.两种神经组织中二氧化碳固定和乙酰辅酶A途径的相对贡献
Nature. 1967 Dec 2;216(5118):928-9. doi: 10.1038/216928a0.
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Acetylcholine synthesis from 14C-labeled substrates in the lobster nerve.龙虾神经中由14C标记底物合成乙酰胆碱
Brain Res. 1967 Jan 20;3(4):384-6. doi: 10.1016/0006-8993(67)90101-1.
6
CO2 fixation in the nervous tissue. IV. CO2 fixation and citrate metabolism in lobster nerve.神经组织中的二氧化碳固定。IV. 龙虾神经中的二氧化碳固定与柠檬酸代谢。
Exp Brain Res. 1966;1(3):284-90 contd. doi: 10.1007/BF00234348.
7
CO2 fixation in the nervous system. V. CO2 fixation and citrate metabolism in rabbit nerve.神经系统中的二氧化碳固定。V. 兔神经中的二氧化碳固定与柠檬酸代谢
Exp Brain Res. 1966;1(3):291-8 contd. doi: 10.1007/BF00234349.
8
Evidence for the metabolic compartmentalization of acetyl-coenzyme A in rat brain slices and its relation to the syntheses of acetylcholine and glutamate.大鼠脑片中乙酰辅酶A代谢区室化的证据及其与乙酰胆碱和谷氨酸合成的关系。
Life Sci. 1969 Jun 15;8(12):657-62. doi: 10.1016/0024-3205(69)90223-9.
9
A study on the precursors of the acetyl moiety of acetylcholine in brain slices. Observations on the compartmentalization of the acetyl-coenzyme A pool.脑切片中乙酰胆碱乙酰基部分前体的研究。关于乙酰辅酶A池分隔的观察。
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10
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