Diebler M F, Morot-Gaudry Y
Biochem J. 1977 Sep 15;166(3):447-53. doi: 10.1042/bj1660447.
Formation of acetyl-CoA through acetyl-CoA synthetase (forward reaction) and through choline acyltransferase (backward reaction) was investigated in tissue extract from the electric organ of Torpedo marmorata. When the tissue extract was submitted to gel filtration on Sephadex G-25, the formation of acetyl-CoA by acetyl-CoA synthetase appeared fully dependent on ATP and CoA and partially dependent on acetate (an endogenous supply of acetate is discussed). Choline acetyltransferase was a potent source of acetyl-CoA, only requiring acetylcholine and CoA, and was much more efficient than acetyl-CoA synthetase for concentrations of acetylcholine likely to be present in nerve endings.
在电鳐（Torpedo marmorata）电器官的组织提取物中，研究了通过乙酰辅酶A合成酶（正向反应）和胆碱酰基转移酶（反向反应）形成乙酰辅酶A的过程。当将组织提取物进行葡聚糖凝胶G-25柱层析时，乙酰辅酶A合成酶形成乙酰辅酶A的过程似乎完全依赖于ATP和辅酶A，部分依赖于乙酸盐（讨论了乙酸盐的内源性供应）。胆碱乙酰转移酶是乙酰辅酶A的一个有效来源，只需要乙酰胆碱和辅酶A，对于神经末梢中可能存在的乙酰胆碱浓度，它比乙酰辅酶A合成酶效率高得多。
