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地衣芽孢杆菌青霉素酶产生过程中的磷脂代谢

Phospholipid metabolism during penicillinase production in Bacillus licheniformis.

作者信息

Morman M R, White D C

出版信息

J Bacteriol. 1970 Oct;104(1):247-53. doi: 10.1128/jb.104.1.247-253.1970.

DOI:10.1128/jb.104.1.247-253.1970
PMID:5473893
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC248207/
Abstract

During membrane-bound penicillinase production, Bacillus licheniformis forms vesicles and tubules that do not appear in the absence of penicillinase production. The major lipids of B. licheniformis were shown to be phospholipids. The proportions, metabolism, and the total phospholipid per cell were shown to be essentially the same in the uninduced control, induced and constitutive penicillinase forming cells during both the exponential and stationary growth phases. Membrane phospholipids were not secreted into the medium during penicillinase formation. In the shift from the exponential to the stationary growth phase, there was an accumulation of phosphatidyl glycerol and a marked decrease in cardiolipin. These two lipids had the most active turnover of their phospholipid phosphate of all the lipids studied.

摘要

在产生膜结合青霉素酶的过程中,地衣芽孢杆菌会形成囊泡和小管,而在不产生青霉素酶时则不会出现。已证明地衣芽孢杆菌的主要脂质是磷脂。在指数生长期和稳定期,未诱导的对照细胞、诱导型和组成型青霉素酶形成细胞中的磷脂比例、代谢以及每个细胞的总磷脂量基本相同。在青霉素酶形成过程中,膜磷脂不会分泌到培养基中。从指数生长期转变到稳定生长期时,磷脂酰甘油会积累,而心磷脂则会显著减少。在所研究的所有脂质中,这两种脂质的磷脂磷酸周转最为活跃。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2653/248207/332d4d2284ac/jbacter00376-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2653/248207/332d4d2284ac/jbacter00376-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2653/248207/332d4d2284ac/jbacter00376-0279-a.jpg

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Phospholipid metabolism during penicillinase production in Bacillus licheniformis.地衣芽孢杆菌青霉素酶产生过程中的磷脂代谢
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2
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J Bacteriol. 1975 Jan;121(1):83-90. doi: 10.1128/jb.121.1.83-90.1975.
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Phospholipid metabolism in the absence of net phospholipid synthesis in a glycerol-requiring mutant of Bacillus subtilis.枯草芽孢杆菌甘油需求突变体中在无净磷脂合成情况下的磷脂代谢
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Bacillus licheniformis penicillinase synthesized in Escherichia coli contains covalently linked fatty acid and glyceride.在大肠杆菌中合成的地衣芽孢杆菌青霉素酶含有共价连接的脂肪酸和甘油酯。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3506-10. doi: 10.1073/pnas.78.6.3506.

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Use of lipid composition and metabolism to examine structure and activity of estuarine detrital microflora.利用脂质组成和代谢来研究河口碎屑微生物区系的结构和活性。
Appl Environ Microbiol. 1977 May;33(5):1177-83. doi: 10.1128/aem.33.5.1177-1183.1977.
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Metabolism of phosphatidylglycerol, phosphatidylethanolamine, and cardiolipin of Bacillus stearothermophilus.

本文引用的文献

1
The measurement of the liberation of penicillinase from Bacillus subtilis.枯草芽孢杆菌青霉素酶释放量的测定。
J Gen Microbiol. 1961 Oct;26:239-53. doi: 10.1099/00221287-26-2-239.
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The location of cell-bound penicillinase in Bacillus subtilis.枯草芽孢杆菌中细胞结合型青霉素酶的定位。
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PURIFICATION AND PROPERTIES OF PENICILLINASES FROM TWO STRAINS OF BACILLUS LICHENIFORMIS: A CHEMICAL, PHYSICOCHEMICAL AND PHYSIOLOGICAL COMPARISON.两株地衣芽孢杆菌青霉素酶的纯化及性质:化学、物理化学和生理学比较
嗜热脂肪芽孢杆菌的磷脂酰甘油、磷脂酰乙醇胺和心磷脂的代谢
J Bacteriol. 1973 Jun;114(3):1125-37. doi: 10.1128/jb.114.3.1125-1137.1973.
Biochem J. 1965 Mar;94(3):666-75. doi: 10.1042/bj0940666.
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COLORIMETRIC ULTRAMICRO METHOD FOR THE DETERMINATION OF FREE FATTY ACIDS.用于测定游离脂肪酸的比色超微量法。
J Lipid Res. 1965 Jul;6:431-3.
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THE DISTRIBUTION AND FORMATION OF PENICILLINASE IN A BACTERIAL POPULATION OF BACILLUS LICHENIFORMIS.地衣芽孢杆菌细菌群体中青霉素酶的分布与形成
J Gen Microbiol. 1964 Mar;34:363-77. doi: 10.1099/00221287-34-3-363.
6
ON THE ACCUMULATION OF AMINO ACID DERIVATIVES OF PHOSPHATIDYLGLYCEROL IN BACTERIA.关于细菌中磷脂酰甘油氨基酸衍生物的积累
Biochim Biophys Acta. 1964 Feb 24;84:96-8. doi: 10.1016/0926-6542(64)90106-4.
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A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
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Intermediates in the biosynthesis of bacterial penicillinase.细菌青霉素酶生物合成的中间体。
Biochem J. 1958 Dec;70(4):665-81. doi: 10.1042/bj0700665.
9
Iodometric assay of penicillinase.青霉素酶的碘量法测定
Nature. 1954 Nov 27;174(4439):1012-3. doi: 10.1038/1741012a0.
10
Release of penicillinase by Bacillus licheniformis.地衣芽孢杆菌释放青霉素酶。
J Gen Microbiol. 1967 Aug;48(2):261-8. doi: 10.1099/00221287-48-2-261.