Citarella R V, Colwell R R
J Bacteriol. 1970 Oct;104(1):434-42. doi: 10.1128/jb.104.1.434-442.1970.
Polynucleotide relationships among selected Vibrio species were examined by means of deoxyribonucleic acid (DNA) reassociation reactions and chromatography on hydroxyapatite. Relative levels of intraspecific DNA duplex formation (V. cholerae-V. cholerae and V. parahaemolyticus-V. parahaemolyticus) were found to be high at 60 C (>80%), and only minimally reduced at 75 C. Interspecific DNA duplexes between V. cholerae DNA and that of the non-cholera vibrios also exhibited high relative levels of formation at 60 C (>80%) and, with one exception, were only slightly reduced at 75 C. The thermal stability of these duplexes formed at 60 or 75 C was virtually identical to that of homologous V. cholerae DNA duplexes. The degree of reassociation and the thermal stability of V. cholerae-non-cholera vibrio DNA duplexes suggests relatively little evolutionary divergence in these organisms. In all other interspecific DNA reassociation reactions, only low levels of DNA duplex formation were noted at 60 C (<25%), and these were drastically reduced (>50%) at 75 C. The degree of nucleotide sequence divergence indicated by these reactions suggests that these Vibrio species are not significantly related to V. cholerae or V. parahaemolyticus. Reassociation reactions between V. cholerae DNA and the DNA of V. parahaemolyticus indicated these species were not significantly related to each other.
通过脱氧核糖核酸（DNA）重结合反应以及在羟基磷灰石上的色谱分析，研究了选定弧菌属物种之间的多核苷酸关系。发现种内DNA双链体形成的相对水平（霍乱弧菌 - 霍乱弧菌以及副溶血性弧菌 - 副溶血性弧菌）在60℃时很高（>80%），在75℃时仅略有降低。霍乱弧菌DNA与非霍乱弧菌DNA之间的种间DNA双链体在60℃时也表现出较高的相对形成水平（>80%），并且除了一个例外，在75℃时仅略有降低。这些在60℃或75℃形成的双链体的热稳定性与同源霍乱弧菌DNA双链体的热稳定性几乎相同。霍乱弧菌 - 非霍乱弧菌DNA双链体的重结合程度和热稳定性表明这些生物体之间的进化差异相对较小。在所有其他种间DNA重结合反应中，在60℃时仅观察到低水平的DNA双链体形成（<25%），而在75℃时这些水平大幅降低（>50%）。这些反应所表明的核苷酸序列差异程度表明这些弧菌属物种与霍乱弧菌或副溶血性弧菌没有显著关系。霍乱弧菌DNA与副溶血性弧菌DNA之间的重结合反应表明这些物种彼此之间没有显著关系。
