白斑角鲨（Squalus acanthias）中促黑素细胞激素的纯化及氨基酸序列
Purification and amino acid sequence of melanocyte-stimulating hormone from the dogfish Squalus acanthias.
作者信息
Lowry P J, Chadwick A
出版信息
Biochem J. 1970 Aug;118(5):713-8. doi: 10.1042/bj1180713.
Abstract
A melanocyte-stimulating hormone (MSH) was isolated by gel filtration and ion-exchange chromatography from extracts of the pituitary glands of dogfish. Sequence studies were carried out on the hormone and its enzymically and chemically cleaved fragments. The sequence of the hormone, Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Met, shows that ten of its 11 residues are the same as ten of the 13 residues of mammalian alpha-MSH. About half of its molecules have the carboxyl group at the C-terminus free and about half are amidated; about a fifth have an extra tyrosine residue on the N-terminus, thereby making 11 residues the same as in mammalian alpha-MSH. Unlike the mammalian hormone, however, none of it was found to be N-acetylated.
摘要
通过凝胶过滤和离子交换色谱法从角鲨的垂体提取物中分离出一种促黑素细胞激素（MSH）。对该激素及其经酶切和化学裂解的片段进行了序列研究。该激素的序列为Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Met，表明其11个残基中的10个与哺乳动物α-MSH的13个残基中的10个相同。其分子约一半的C末端羧基是游离的，约一半是酰胺化的；约五分之一在N末端有一个额外的酪氨酸残基，从而使11个残基与哺乳动物α-MSH中的相同。然而，与哺乳动物激素不同的是，未发现其有N-乙酰化。
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