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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochem J. 1970 Aug;118(5):851-7. doi: 10.1042/bj1180851.

A method is described for the extraction, purification and separation of acid-soluble phosphorus compounds from mammalian semen. [8-(14)C]ATP and [8-(14)C]AMP were used as internal recovery standards to measure the breakdown and loss of these nucleotides in the procedure. 2. Bull, ram, boar and stallion semen was separated into seminal plasma and spermatozoa and the two fractions were examined separately. The overall composition of the mixture of the phosphorus compounds extracted from the two fractions was similar for the four species. 3. Glycerylphosphorylcholine and glycerylphosphorylinositol were the two phosphorus compounds identified in extracts of seminal plasma. ATP, ADP, AMP, GTP, GDP, NAD, fructose 1,6-diphosphate and glucose 6-phosphate were identified in extracts prepared from spermatozoa.

本文描述了一种从哺乳动物精液中提取、纯化和分离酸溶性磷化合物的方法。使用[8-(14)C]ATP和[8-(14)C]AMP作为内部回收率标准，以测量这些核苷酸在该过程中的分解和损失。2. 将公牛、公羊、公猪和种马的精液分离为精浆和精子，并分别对这两个部分进行检查。从这两个部分提取的磷化合物混合物的总体组成在这四个物种中相似。3. 甘油磷酰胆碱和甘油磷酰肌醇是在精浆提取物中鉴定出的两种磷化合物。在从精子制备的提取物中鉴定出了ATP、ADP、AMP、GTP、GDP、NAD、果糖1,6-二磷酸和葡萄糖6-磷酸。

Biochem J. 1970 Aug;118(5):851-7. doi: 10.1042/bj1180851.

A method is described for the extraction, purification and separation of acid-soluble phosphorus compounds from mammalian semen. [8-(14)C]ATP and [8-(14)C]AMP were used as internal recovery standards to measure the breakdown and loss of these nucleotides in the procedure. 2. Bull, ram, boar and stallion semen was separated into seminal plasma and spermatozoa and the two fractions were examined separately. The overall composition of the mixture of the phosphorus compounds extracted from the two fractions was similar for the four species. 3. Glycerylphosphorylcholine and glycerylphosphorylinositol were the two phosphorus compounds identified in extracts of seminal plasma. ATP, ADP, AMP, GTP, GDP, NAD, fructose 1,6-diphosphate and glucose 6-phosphate were identified in extracts prepared from spermatozoa.

本文描述了一种从哺乳动物精液中提取、纯化和分离酸溶性磷化合物的方法。使用[8-(14)C]ATP和[8-(14)C]AMP作为内部回收率标准，以测量这些核苷酸在该过程中的分解和损失。2. 将公牛、公羊、公猪和种马的精液分离为精浆和精子，并分别对这两个部分进行检查。从这两个部分提取的磷化合物混合物的总体组成在这四个物种中相似。3. 甘油磷酰胆碱和甘油磷酰肌醇是在精浆提取物中鉴定出的两种磷化合物。在从精子制备的提取物中鉴定出了ATP、ADP、AMP、GTP、GDP、NAD、果糖1,6-二磷酸和葡萄糖6-磷酸。