Muir G G, Ryan M, Conaill D U
J Clin Pathol. 1970 Sep;23(6):492-8. doi: 10.1136/jcp.23.6.492.
An automated assay suitable for estimating urinary oestrogens in pregnant women has been investigated. Fluorimetry was found to have considerable advantages over colorimetry. The fluorimetric assay was simpler, more precise, more sensitive, and eliminated the need for correction for non-specific chromogens; in the assay of oestriol in pregnant women there was no need for correction for non-specific fluorescence. Spectrofluorimetric and photometric analyses, recoveries, and reproducibility show that the method offers a robust means of providing values for urinary oestrogen in pregnant women on a scale of up to 100 tests a day, the time of the assay being one and a half hours.
人们对一种适用于估算孕妇尿中雌激素的自动化检测方法进行了研究。结果发现，荧光测定法比比色法具有相当多的优势。荧光测定法更简单、更精确、更灵敏，并且无需对非特异性色原进行校正；在检测孕妇尿中雌三醇时，无需对非特异性荧光进行校正。荧光分光光度法和光度分析、回收率及重现性表明，该方法提供了一种可靠的手段，能够以每天最多100次检测的规模为孕妇尿中雌激素提供数值，检测时间为一个半小时。
