聚阳离子对肝素摄取和脱硫作用的差异效应

Differential effect of polycations on uptake and desulphation of heparin.

作者信息

Fabian I, Bleiberg I, Aronson M

出版信息

Adv Exp Med Biol. 1979;121(A):175-82. doi: 10.1007/978-1-4684-3593-1_16.

Abstract

Previous work has established that macrophages in culture release sulphate from heparin. We now report that increased uptake and desulphation of heparin occurred in the presence of polycations (poly-L-ornithine and poly-DL-lysine) and that the increase in heparin uptake was by about 30-fold. The desulphation was less related to uptake than to the nature of the bound polycation. Serum was found to have an inhibitory effect on heparin uptake while polycations inhibited heparin desulphation by macrophage extracts.

摘要

先前的研究已经证实，培养的巨噬细胞会从肝素中释放出硫酸盐。我们现在报告，在聚阳离子（聚-L-鸟氨酸和聚-DL-赖氨酸）存在的情况下，肝素的摄取和去硫酸化会增加，并且肝素摄取的增加约为30倍。去硫酸化与摄取的关系较小，而与结合的聚阳离子的性质有关。发现血清对肝素摄取有抑制作用，而聚阳离子抑制巨噬细胞提取物对肝素的去硫酸化作用。