A technique for detection of agglutinating activity of antilymphocytic serum using formalinized and trypan-blue-stained lymphocytes.

This paper describes technical details of a micro-reaction for the detection of agglutinating potency of antilymphocytic serum produced in rabbits with chicken, rat and dog thymus cells. The titration of antilymphocytic serum includes the use of microtitre plastic plates and lymphocytes treated with formalin and stained with trypan blue stain. Formalinized and stained lymphocytes represent a stable antigen of long durability, and the application of those cells in leucoagglutination increases the accuracy and sensitivity of the reaction.