监测甾体微生物转化的分光光度法。I. 发酵液中9-α-氟氢化可的松和9-α-氟-16-α-羟基氢化可的松的测定
Spectrophotometric methods for monitoring the microbial transformation of steroids. I. Determination of 9-alpha-fluorohydrocortisone and 9-alpha-fluoro-16-alpha-hydroxyhydrocortisone in fermentation broths.
作者信息
Ivashkiv E
出版信息
Appl Microbiol. 1970 Aug;20(2):251-3. doi: 10.1128/am.20.2.251-253.1970.
Abstract
A method has been devised for the determination of 9alpha-fluorohydrocortisone and 9alpha-fluoro-16alpha-hydroxyhydrocortisone in fermentation broths. The method involves extraction of the two steroids from the broth with ethyl acetate, separation through the formation of the water-soluble borate complex of the vicinally hydroxylated steroid, and estimation of each steroid spectrophotometrically.
摘要
已设计出一种用于测定发酵液中9α-氟氢化可的松和9α-氟-16α-羟基氢化可的松的方法。该方法包括用乙酸乙酯从发酵液中萃取这两种甾体，通过形成邻位羟基化甾体的水溶性硼酸盐络合物进行分离，并用分光光度法对每种甾体进行测定。
相似文献
1
Spectrophotometric methods for monitoring the microbial transformation of steroids. I. Determination of 9-alpha-fluorohydrocortisone and 9-alpha-fluoro-16-alpha-hydroxyhydrocortisone in fermentation broths.监测甾体微生物转化的分光光度法。I. 发酵液中9-α-氟氢化可的松和9-α-氟-16-α-羟基氢化可的松的测定
Appl Microbiol. 1970 Aug;20(2):251-3. doi: 10.1128/am.20.2.251-253.1970.
2
16alpha-Hydroxy steroids. IX. Effect of medium composition on isomerization of 9alpha-fluoro-16alpha-hydroxyhydrocortisone and 9alpha-fluoro-16alpha-hydroxyprednis-olone (triamcinolone) during microbiological fermentations.16α-羟基类固醇。IX. 培养基成分对微生物发酵过程中9α-氟-16α-羟基氢化可的松和9α-氟-16α-羟基泼尼松龙（曲安西龙）异构化的影响。
Appl Microbiol. 1960 Nov;8(6):363-6. doi: 10.1128/am.8.6.363-366.1960.
3
Spectrophotometric methods for monitoring the microbial transformation of steroids. II. Absorbance ratio spectrophotometric determination of per cent conversion of 9 -fluoro-16 -hydroxyhydrocortisone to 9 -fluoro-16 -hydroxyprednisolone.监测甾体微生物转化的分光光度法。II. 9-氟-16-羟基氢化可的松转化为9-氟-16-羟基泼尼松龙转化率的吸光度比分光光度法测定
Biotechnol Bioeng. 1971 Jul;13(4):561-7. doi: 10.1002/bit.260130409.
4
16alpha-Hydroxy steroids. XI. 2beta-and 16alpha-hydroxylation of 9alpha-fluorohydrocortisone by strains of Streptomyces roseochromogenes.16α-羟基类固醇。十一。玫瑰色链霉菌菌株对9α-氟氢化可的松的2β-和16α-羟基化作用
Appl Microbiol. 1961 Sep;9(5):372-5. doi: 10.1128/am.9.5.372-375.1961.
5
Steroid borates. I. Structural considerations.类固醇硼酸盐。一、结构方面的考虑。
J Pharm Sci. 1961 Mar;50:193-7. doi: 10.1002/jps.2600500303.
6
[Metabolic effect of metacortandracin and of 9-alpha-fluorohydrocortisone acetate].[间甲去氢氢化可的松和9-α-氟氢化可的松醋酸酯的代谢作用]
Reumatismo. 1955 May-Jun;7(3):127-37.
7
Further experiences with delta 1,9 alpha fluoro, 16 alpha hydroxyhydrocortisone (triamcinolone) in treatment of patients with rheumatoid arthritis.1,9α-氟-16α-羟基氢化可的松（曲安奈德）治疗类风湿关节炎患者的更多经验
Arthritis Rheum. 1958 Jun;1(3):215-29. doi: 10.1002/art.1780010304.
8
Facile differential UV determination of steroids with conjugated ketone chromophores via lithium borohydride reduction.通过硼氢化锂还原，利用共轭酮发色团对甾体进行简便的差示紫外测定。
J Pharm Sci. 1972 Jan;61(1):148-9. doi: 10.1002/jps.2600610142.
9
Acute effects of 9alpha-fluorohydrocortisone and 2-methyl-9alpha-fluorohydrocortisone on renal clearances of sodium and potassium in man.9α-氟氢化可的松和2-甲基-9α-氟氢化可的松对人体钠和钾肾清除率的急性影响。
Proc Soc Exp Biol Med. 1961 Dec;108:592-7. doi: 10.3181/00379727-108-27006.
10
Downstream Process Synthesis for Microbial Steroids.微生物甾体的下游工艺合成
Methods Mol Biol. 2017;1645:321-345. doi: 10.1007/978-1-4939-7183-1_23.
本文引用的文献
1
Transformation of steroids by spores of microorganisms. I. Hydroxylation of progesterone by conidia of Aspergillus ochraceus.微生物孢子对甾体的转化。I. 赭曲霉分生孢子对孕酮的羟基化作用。
Appl Microbiol. 1963 Jan;11(1):50-7. doi: 10.1128/am.11.1.50-57.1963.
2
Steroid borates. I. Structural considerations.类固醇硼酸盐。一、结构方面的考虑。
J Pharm Sci. 1961 Mar;50:193-7. doi: 10.1002/jps.2600500303.
3
16alpha-Hydroxy steroids. XI. 2beta-and 16alpha-hydroxylation of 9alpha-fluorohydrocortisone by strains of Streptomyces roseochromogenes.16α-羟基类固醇。十一。玫瑰色链霉菌菌株对9α-氟氢化可的松的2β-和16α-羟基化作用
Appl Microbiol. 1961 Sep;9(5):372-5. doi: 10.1128/am.9.5.372-375.1961.
Zotero 插件