Sebastiani L, Tricarico L, Pitzurra L, Franceschini S
Boll Soc Ital Biol Sper. 1979 Oct 30;55(20):2105-10.
Trypsinized cells from rabbit kidney can be kept at -195, 8 degrees C indefinitely. When necessary, propagation in monolayer cultures is readily obtained as primary cultures, provided that some conditions be respected: 1) only glass culture flask must be used. Falcon plastic vessels do not allow cell attachment in this type of primary culture 2) the lag period is particularly long: it taken about 20 days before the beginning of cell multiplication 3) fetal calf serum is toxic for the cells in the 2nd and 3rd change of the culture medium, during the lag period.
来自兔肾的胰蛋白酶消化细胞可在-195.8℃无限期保存。必要时，只要遵循一些条件，作为原代培养很容易获得单层培养的细胞增殖：1）必须仅使用玻璃培养瓶。在这种原代培养类型中，Falcon塑料容器不允许细胞附着；2）延迟期特别长：细胞增殖开始前大约需要20天；3）在延迟期，胎牛血清在培养基的第二次和第三次更换时对细胞有毒。
