高等植物中由L-古洛糖酸-1,4-内酯和L-半乳糖酸-1,4-内酯合成L-抗坏血酸
L-ascorbic acid biosynthesis in higher plants from L-gulono-1, 4-lactone and L-galactono-1, 4-lactone.
作者信息
Baig M M, Kelly S, Loewus F
出版信息
Plant Physiol. 1970 Aug;46(2):277-80. doi: 10.1104/pp.46.2.277.
Abstract
Detached bean (Phaseolus vulgaris) and strawberry (Fragaria) fruits fed l-gulono-1,4-lactone or l-galactono-1,4-lactone convert this compound, in part, to l-ascorbic acid. When l-galactono-1,4-lactone is given as a 0.25% solution to detached bean shoots, the ascorbic acid content is tripled in less than 10 hours. l-Gulono-1,4-lactone is only 5 to 10% as effective as its epimer. Experiments with specifically labeled l-gulono-1,4-lactone and l-galactono-1,4-lactone prove that conversion is direct. Ascorbic acid is labeled at the same carbon as its precursor.A method is described for preparation of l-galactono-1,4-lactone-2-(14)C from myo-inositol-2-(14)C. This method can be extended to the preparation of l-ascorbic acid-2-(14)C on the basis of results obtained in the present study.
摘要
给离体的菜豆（菜豆属）和草莓（草莓属）果实喂食L-古洛糖酸-1,4-内酯或L-半乳糖酸-1,4-内酯，它们会将这种化合物部分转化为L-抗坏血酸。当以0.25%的溶液形式给离体的菜豆嫩枝喂食L-半乳糖酸-1,4-内酯时，抗坏血酸含量在不到10小时内增加两倍。L-古洛糖酸-1,4-内酯的效果仅为其差向异构体的5%至10%。用经过特定标记的L-古洛糖酸-1,4-内酯和L-半乳糖酸-1,4-内酯进行的实验证明，转化是直接的。抗坏血酸在与它的前体相同的碳原子上被标记。描述了一种从肌醇-2-(14)C制备L-半乳糖酸-1,4-内酯-2-(14)C的方法。根据本研究获得的结果，该方法可扩展用于制备L-抗坏血酸-2-(14)C。
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