Miller R J, Dumford W S, Koeppe D E
Plant Physiol. 1970 Sep;46(3):471-4. doi: 10.1104/pp.46.3.471.
The effects of gramicidin D, S, and J on corn mitochondria respiration and swelling were studied. Only gramicidin D was found to have any pronounced effect on mitochondrial swelling. In buffered KCl gramicidin D produced a rapid, respiration-independent swelling which was not reversed with respiratory inhibitors or substrate exhaustion. The respiration rate of exogenous reduced nicotinamide adenine dinucleotide was stimulated by all three gramicidins, but the effects on malate-pyruvate and succinate respiration depended on the type of gramicidin and the reaction media. The respiration effects of gramicidin D may be due to action at specific sites for each substrate.
研究了短杆菌肽D、S和J对玉米线粒体呼吸及肿胀的影响。结果发现只有短杆菌肽D对线粒体肿胀有显著作用。在缓冲的氯化钾溶液中,短杆菌肽D能引起快速的、与呼吸无关的肿胀,这种肿胀不会因呼吸抑制剂或底物耗尽而逆转。所有三种短杆菌肽均能刺激外源还原型烟酰胺腺嘌呤二核苷酸的呼吸速率,但对苹果酸 - 丙酮酸和琥珀酸呼吸的影响取决于短杆菌肽的类型和反应介质。短杆菌肽D对呼吸的影响可能是由于其作用于每种底物的特定位点。