大鼠肠黏膜中溶血卵磷脂的酰化作用。
Acylation of lysolecithin in the intestinal mucosa of rats.
作者信息
Subbaiah P V, Sastry P S, Ganguly J
出版信息
Biochem J. 1970 Jun;118(2):241-6. doi: 10.1042/bj1180241.
Abstract
- The presence of an active acyl-CoA-lysolecithin (1-acylglycerophosphorylcholine) acyltransferase was demonstrated in rat intestinal mucosa. 2. ATP and CoA were necessary for the incorporation of free [1-(14)C]oleic acid into lecithin (phosphatidylcholine). 3. The reaction was about 20 times as fast with [1-(14)C]oleoyl-CoA as with free oleic acid, CoA and ATP. 4. With 1-acylglycerophosphorylcholine as the acceptor, both oleic acid and palmitic acid were incorporated into the beta-position of lecithin; the incorporation of palmitic acid was 60% of that of oleic acid. 5. Of the various analogues of lysolecithin tested as acyl acceptors from [1-(14)C]oleoyl CoA, a lysolecithin with a long-chain fatty acid at the 1-position was most efficient. 6. The enzyme was mostly present in the brush-border-free particulate fraction of the intestinal mucosa. 7. Of the various tissues of rats tested for the activity, intestinal mucosa was found to be the most active, with testes, liver, kidneys and spleen following it in decreasing order.
摘要
- 在大鼠肠黏膜中证实存在活性酰基辅酶A - 溶血卵磷脂（1 - 酰基甘油磷酸胆碱）酰基转移酶。2. 游离的[1 - （14）C]油酸掺入卵磷脂（磷脂酰胆碱）需要ATP和辅酶A。3. 以[1 - （14）C]油酰辅酶A作为底物时，该反应速度比以游离油酸、辅酶A和ATP作为底物时快约20倍。4. 以1 - 酰基甘油磷酸胆碱作为受体时，油酸和棕榈酸均掺入卵磷脂的β位；棕榈酸的掺入量为油酸的60%。5. 在测试的作为[1 - （14）C]油酰辅酶A酰基受体的各种溶血卵磷脂类似物中，1位带有长链脂肪酸的溶血卵磷脂效率最高。6. 该酶主要存在于肠黏膜无刷状缘的颗粒部分。7. 在测试的大鼠各种组织的活性中，发现肠黏膜活性最高，睾丸、肝脏、肾脏和脾脏依次递减。
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